This Build a Bioreactor Kit guides students through the design and manufacture of a bioreactor which can then be used to process milk or whey using an immobilised enzyme. The basis of the experiment is the hydrolysis of lactose found in milk. The reaction is monitored using diabetic urine testing strips which give an indication of the concentration of glucose present. Student worksheets give an outline of the required features of a bioreactor - the rest is up to you! The teacher's notes give background information and guide-lines on how to use the kit. Detailed instructions of how to immobilise the enzyme, and ideas for further investigations are also included. The kit includes:. Plastic Bottles. Filter Funnels. 3-Way Taps. T-Connectors. Syringes. Silicone Tubing. Glass Tubing. Rubber Bings with 1 hole. Sieve. Gauze. Glucose Testing Strips. Lactase (B-Galactosidase). Calcium Chloride. Sodium Alginate. Sufficient materials for five students/groups. In addition to the included materials, student groups will also need the following:. 200ml UHT milk. Distilled water. Photocopies of the included worksheet. 2 Beakers. Glass stirring rod. 100ml measuring cylinder. Aquarium Pump and some means of regulating the air flow e.g. air tap. Hot glue gun. Scissors. Soldering Iron. Retort Stand with 2 bosses and clamps.
