This Human DNA Typing Kit allows your students to use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype! They can then compare their class results with others around the world over the internet. Group Size: for 25 students. Storage: Some components require freezer storage on receipt. Time Required: Extraction - 50 minutes, PCR set-up - 10 minutes, PCR - 90 minutes, Electrophoresis - 60 minutes, staining - 5 minutes to overnight. The kit includes:. Full Instructions. PCR EdvoBeads™ PLUS. PV92 LyphoPrimer™ Mix. EdvoQuick™ DNA Ladder. LyphoControl™. Universal DNA Buffer. TE Buffer. Proteinase K. UltraSpec-Agarose™. TBE Electrophoresis Buffer Powder. SYBR® Safe Stain. FlashBlue™ Stain. Microcentrifuge & PCR Tubes. Disposable Plastic Cups. Salt Packets. a 15 mL conical tube. Additional required materials (not included with the kit):. DNA Electrophoresis Tank, with accessories including casting trays, micropipettes & special power supply (B8R03273). Thermal Cycler (B8R07450). UV or Blue Light Transilluminator White Light Box - optional (B8R07358). 2 x Water Bath (B8R06048). Microcentrifuge (B8R04574). Microwave or Hot plate.
