Some foods contain raw materials from genetically modified organisms (GMO). Examples include tofu, corn flakes and corn meal. In this Identification of Genetically Modified Food Kit, your students will extract DNA from food or plant material and perform PCR to determine if any GM indicator genes are present. Amplified DNA is separated and sized by agarose gel electrophoresis. Group Size: for 10 lab groups. Storage: some components require freezer storage upon receipt. Time Required: Extraction - 45 minutes, PCR set-up - 10 minutes, PCR - 2hours, Electrophoresis - 60mins, Staining - 5 minutes. The kit includes:. Full Instructions. PCR EdvoBeads™. Universal DNA Buffer. TE Buffer. LyphoPrimer™ Mix. GMO Positive LyphoControl™. NaCl Solution. DNA Extraction Buffer. 100 base pair ladder,. Proteinase K. UltraSpec Agarose™. TBE Electorphoresis buffer powder. SYBR® Safe Stain. 0.2ml PCR Tubes. Plastic micropestles. Transfer pipettes. Snap-top microcentrifuge tubes. Additional required equipment & materials (not supplied with the kit):. DNA Electrophoresis Tank, with accessories including casting trays, micropipettes & special power supply (B8R03273). Thermal Cycler (B8R07450). UV or Blue Light Transilluminator (B8R07449). Waterbath (B8R06048). Microcentrifuge (B8R04574). Microwave or hot plate. Isopropanol (B8F78836). 70% Ethanol (B8A67749).
